FIG 1.

Genome gene discovery and pangenome. (A) To evaluate the completeness of the gene-finding effort, a rarefaction plot was constructed by listing the COG to which each V. parahaemolyticus isolate belonged, resampling the isolates with replacement using random.randrange in Python 3.6, and then listing and counting the unique COGs. This was done for increasing numbers of isolates. For each number of isolates, 10 replicates were done. The plot was constructed with Plotnine, geom_point. (B) The pangenome was constructed to demonstrate a core genome with a highly variable auxiliary genome.