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RhoA/ROCK1 pathway regulated the interaction of myosin IIA and IIB with actin in human NP cells. (A, B) Human NP cells untreated or pretreated with 20 μM Y27632 for 2 h prior to compression stress for 36 h were stained with myosin IIA or IIB (green), F‐actin (red) and DAPI (blue). Scale bar: 5 μm. The co‐localization of myosin IIA or IIB with F‐actin was evaluated on the basis of Pearson coefficients. Data were presented as the mean ± SD (n = 3). *P < .05, vs compression
