Figure 2.

IL‐33 skews M2 macrophage polarization. A, Expression of CD206 determined by flow cytometry in IL‐33–induced macrophage increased with increasing IL‐33 concentration. B‐E), IL‐10, TGF‐β, IL‐12p35 and CD206 determined by qPCR in IL‐33–induced macrophage increased with increasing IL‐33 concentration. F, Immunofluorescence staining for IL‐33–induced M2 macrophages (scale bar = 20 μm). The green signal represents the staining of activated ornithine decarboxylase, and the blue signal represents the DAPI‐stained nuclei. *P < .05; **P < .01; ***P < .001. Bars indicate mean and SEM of triplicate experiments and show a representative experiment of at least 3 independent experiments performed for each panel