Figure 5.

Supernatants from IL‐33–induced M2 macrophages promote ESCC cells proliferation and migration. A, The proliferation of ECA109 cells co‐cultured with the supernatants from M2‐type macrophages with or without knocking down ornithine decarboxylase (ODC), and OD450 values were measured to evaluate the proliferation. OD450 values are presented as the mean ± SEM, n = 5, P < .05. B, Migration abilities of the ECA109 cells co‐cultured with supernatants from M2‐type macrophages with or without knocking down ODC were determined by wound‐healing assay and Transwell assay. Representative images were taken at 0 and 48 h in wound‐healing assay (scale bar = 200 μm). C, Representative images of the migration of ECA‐109 cells in Transwell assay and the numbers of migrated cells are presented as the mean ± SEM (scale bar = 50 μm). *P < .05. Bars indicate mean and SEM of triplicate experiments and show a representative experiment of at least 3 independent experiments performed for each panel