FIGURE 4.

Relative expression of lnc‐RHL, and hepatocyte and cholangiocyte markers in undifferentiated and differentiated HepaRG cells. Cells were harvested from 3 independent cultures at days 14 and 33 and assessed by qRT‐PCR for lnc‐RHL, the bipotent hepatic progenitor cell marker α‐fetoprotein (AFP), the mature hepatocyte markers albumin, HNF4α and 11q23.3 hepatocyte‐specific apolipoprotein mRNAs APOA1, APOC3 and APOA5, and the cholangiocyte cytokeratin markers CK7 and CK19. ZPR1 is an additional 11q23.3 transcript not thought to be involved in lipid and cholesterol metabolism. Statistical significance of differential mRNA content is indicated with asterisks: *P < .05, **P < .01 and ***P < .001. Means and error bars were determined from three sample replicates (n = 3)