FIGURE 6.

Effects of lnc‐RHL knockdown upon hepatocyte and cholangiocyte‐specific gene expression. qRT‐PCR assays were performed and standardized to β‐actin expression under the 3 experimental conditions (see Figure 5). lnc‐RHL was extensively knocked down using sh‐B and to a greater extent with sh‐C, either by terminal Dox induction (days 30‐30) or continuously during differentiation (days 14‐33). lnc‐RHL knockdown resulted in cultures with increased expression of AFP, but reduced expression of hepatocyte markers albumin, HNF4α, APOA1, APOC3 and APOA5. In addition, the expression of CK7 was increased in lnc‐RHL knockdown cultures while the expression of CK19 was reduced. The expression of the non‐APO 11q23.3 gene ZPR1 was unaffected. Statistical significance of differential mRNA content is indicated with asterisks: *P < .05, **P < .01 and ***P < .001. Means and error bars were determined from six sample replicates (n = 6)