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. 2021 Jan 20;65(2):e01592-20. doi: 10.1128/AAC.01592-20

FIG 4.

FIG 4

Secreted-protein profiles from ΔycbB mutants of C. rodentium and S. Typhimurium. (A) Secreted-protein profiles from C. rodentium strain DBS100, which utilizes a LEE (locus of enterocyte effacement)-encoded T3SS. The wild-type (wt) strain was used as a positive control, and ΔescC and ΔetgA mutant strains were used as negative controls. The ΔycbB strain is seen to retain LEE T3SS activity. (B) Secreted-protein profiles from S. Typhimurium strain SL1344, which utilizes an SPI-1 (Salmonella pathogenicity island 1)-encoded T3SS. The wild-type (wt) strain was used as a positive control, and the ΔinvG mutant strain was used as a negative control. The ΔycbB strain is seen to retain SPI-1 T3SS activity. The two ΔycbB lanes in each of panels A and B represent two independent isolates, which should be genetically identical. Lanes MW are molecular weight markers.