TABLE 1.
Characteristics of the isolatesa
| Characteristic | Value |
||||
|---|---|---|---|---|---|
| KP15ZE215 | KP15ZE495 | KQBSI104 | Transconjugant | E. coli J53Azr | |
| Source | Human | Human | Human | ||
| Isolation site | Urine | Urine | Blood | ||
| Yr of isolation | 2012 | 2015 | 2017 | ||
| MLST type | 3447 | 3447 | |||
| Plasmid replicon types | FIB, FII, FIA, X4 | FIB, FII, FIA, X4 | FIB, FII, HI1B | ||
| MIC (MIC with NMP) (μg/ml) | |||||
| Tigecycline (+NMP) | 32 (4) | 32 (4) | 16 (1) | 4 (0.25) | 0.25 (0.125) |
| Tetracycline (+NMP) | 64 (16) | 64 (16) | >128 (32) | 128 (32) | ≤1 (≤1) |
| Chloramphenicol (+NMP) | 64 (16) | 64 (16) | >128 (64) | 128 (32) | 8 (4) |
| Ciprofloxacin (+NMP) | >256 (256) | >256 (256) | 32 (16) | 32 (16) | ≤0.03 (≤0.03) |
| Colistin (+NMP) | 64 (64) | 64 (64) | 1 (≤0.25) | 0.25 (≤0.25) | 0.25 (0.25) |
| Aztreonam (+NMP) | 128 (64) | 128 (128) | 64 (64) | 16 (16) | 0.25 (0.25) |
| Cefepime (+NMP) | 128 (64) | 128 (64) | 32 (32) | 32 (32) | ≤0.5 (≤0.5) |
| Ceftazidime (+NMP) | 64 (32) | 64 (32) | 32 (32) | 32 (32) | ≤1 (≤1) |
| Gentamicin (+NMP) | >256 (>256) | >256 (>256) | >256 (>256) | >256 (>256) | 1 (2) |
| Amikacin (+NMP) | >256 (>256) | >256 (>256) | >256 (>256) | >256 (>256) | 1 (2) |
| Cefotaxime (+NMP) | 128 (64) | 128 (128) | 64 (64) | 16 (32) | ≤1 (≤1) |
| Trimethoprim-sulfamethoxazole (+NMP) | 2 (0.5) | 2 (0.5) | >16 (>16) | >16 (>16) | ≤0.25 (≤0.25) |
| Imipenem | 1 | 2 | 0.5 | 0.5 | 0.5 |
| Meropenem | ≤0.25 | ≤0.25 | ≤0.25 | ≤0.25 | ≤0.25 |
a
MLST, multilocus sequence typing. Note that 1-(1-naphthylmethyl)-piperazine (NMP) was added at a concentration of 100 μg/ml.