TABLE 4.
In vitro effect of RDV and its metabolites GS-704277 and GS-441524 on mitochondrial respiration after 3-day treatment in PC-3, HepG2, PHH, and RPTECs
| Compound | Cell model | 3-day CC50 (μM)a |
||
|---|---|---|---|---|
| Spare respiration | Total DNA content | Cellular ATP | ||
| RDV | PC-3 | 2.5 ± 0.1 | 12.5 ± 0.7 | 24.0 ± 1.4 |
| HepG2 | 10.6 ± 0.1 | 6.3 ± 0.9 | 7.9 ± 0.1 | |
| PHH | 7.6 ± 1.9 | 13.4 ± 1.7 | 7.8 ± 2.2 | |
| RPTECs | 7.3 ± 2.7 | 14.3 ± 3.3 | 16.9 ± 4.1 | |
| GS-441524 | PC-3 | >100 | >100 | >100 |
| HepG2 | >100 | >100 | >100 | |
| PHH | >100 | >100 | >100 | |
| RPTECs | >100 | >100 | >100 | |
| GS-704277 | PHH | >100 | >100 | >100 |
| Control chloramphenical | PC-3 | 4.8 ± 1.3 | >50 | >50 |
| Control phenformin | PHH | 1.9 ± 0.5 | 22.1 ± 1.9 | 9.7 ± 4.5 |
a
CC50 values represent the average ± SD from three or more independent experiments.