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. Author manuscript; available in PMC: 2021 Dec 7.
Published in final edited form as: Anticancer Res. 2020 Dec 7;40(12):6637–6647. doi: 10.21873/anticanres.14688

Figure 1.

Figure 1.

Cytotoxicity of (+)-betulin in MDA-MB-231 breast cancer cells. (A) Structure of (+)-betulin; (B) representative microscopic (20x) morphology view of MDA-MB-231 cells after 48 and 72 h of treatment with (+)-betulin (15 μM) or control (DMSO 0.2%). (C) Cytotoxic effects of co-treatment of (+)-betulin and TNFα in MDA-MB-231 cells, using SRB assay. Cells were treated for 48 and 72 h either with (+)-betulin (5 μM) or TNFα (50 ng/ml) or both. Data are expressed as the means±SEM (n=9) and analyzed by one-way ANOVA, using Dunnett’s multiple-comparison test (*p<0.05, ***p<0.01).