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(A) Effect of (+)-betulin on MTP in MDA-MB-231 cells. Cells were treated with (+)-betulin (0–50 μM) or staurosporine (Sta, 8 μM) for 5 h. (B) Western blot analysis of (+)-betulin on Bcl-2 protein levels. Cells were treated 5 h with (+)-betulin (0–50 mM) or rocaglamide (positive control (3μM)). All data are expressed as the means±SEM (n=3) and analyzed by one-way ANOVA using Dunnett’s multiple-comparison test compared to control; *p<0.05, **p<0.01, ***p<0.001.
