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. 2021 Jan 18;7:589080. doi: 10.3389/fmed.2020.589080

Table 2.

Potential false-positive and false-negative rates after heat-inactivation for SARS-CoV-2 IgG and IgM Ab detection with indirect immunity-based kit produced by manufacturer A.

IgG Ab 56°C for 30 min Total 56°C for 45 min Total 56°C for 60 min Total 60°C for 30 min Total 65°C for 30 min Total
Po Ne Po Ne Po Ne Po Ne Po Ne
Control Po 125 (100.0%) 0 (0.0%) 125 125 (100.0%) 0 (0.0%) 125 125 (100.0%) 0 (0.0%) 125 124 (99.2%) 1 (0.8%) 125 121 (96.8%) 4 (3.2%) 125
Ne vitchu0 (0.0%) 4 (100.0%) 4 vitchu0 (0.0%) 4 (100%) 4 vitchu1 (25.0%) 3 (75.0%) 4 vitchu1 (25.0%) 3 (75.0%) 4 vitchu0 (0.0%) 4 (100.0%) 4
Total 125 4 129 125 4 129 126 3 129 125 4 129 121 8 129
IgM Ab Po Ne Total Po Ne Total Po Ne Total Po Ne Total Po Ne Total
Control Po 62 (83.8%) 12 (16.2%) 74 62 (84.0%) 12 (16.0%) 74 61 (82.4%) 13 (17.6%) 74 64 (86.5%) 10 (13.5%) 74 56 (75.7%) 18 (24.3%) 74
Ne 16 (29.1%) 39 (70.9%) 55 11 (21.4%) 44 (78.6%) 55 12 (21.8%) 43 (78.2%) 55 17 (30.9%) 38 (69.1%) 55 16 (29.1%) 39 (70.9%) 55
Total 78 51 129 73 56 129 73 56 129 81 48 129 72 57 129

blue bold, false-negative; red bold, false-positive; Po, positive; Ne, negative.