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. 2021 Jan 31;9:35. doi: 10.1186/s40168-020-00985-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Taxonomic annotation of the two keystone functions. a Relative abundances of the 8 genera for “nitrogen metabolism” and 44 genera for “phosphonate and phosphinate metabolism” across soil pH and dilution levels, based on shotgun metagenomic data. The relative abundances of genera at each row were normalized by removing the mean and dividing by the standard deviation. The color from green to white represents a relative abundance of each genus from high to low. Five columns at each dilution level within the heatmap indicate the soil pH range of 4.5 (left) to 8.5 (right). The genera are colored by phylum on the left side. b The relative abundances of the corresponding genera based on shotgun metagenomic data. Error bars represent standard deviations (SD, n = 60)