FIGURE 2.

NLRP3 deficiency prevents IL‐1β release and inhibits osteoclast differentiation. (A‐D) OCPs from NLRP3^KO and WT mice were cultured and stimulated with or without LPS ± Nigericin during culture, as indicated in the figures. (A) Western blot of cell lysates and supernatants from OCP cultures. (B) Production of IL‐1β from OCPs stimulated with or without LPS ± Nigericin was measured by ELISA. (C) OCPs were cultured with M‐CSF and RANKL and stained for TRAP activity. TRAP⁺ OC number per well was assessed. (D) The expression levels of OC‐related genes were determined by qPCR. All the results are representative of at least three independent experiments. (E) OCPs from NLRP3^KO and WT mice were cultured and stimulated with or without LPS + Nigericin ±Anti‐IL‐1β during culture. OCPs were cultured with M‐CSF and RANKL and stained for TRAP activity. TRAP⁺ OC number per well was assessed. Values are mean ± SD Two‐tailed unpaired Student's t‐test or One‐way ANOVA followed by Dunnett's post‐hoc multiple comparisons was performed. *P < .05 vs Veh or in the indicated groups, #P < .05 vs WT treated with the same reagent