FIGURE 5.

MCC950 inhibits Lysm‐Cre⁺ osteoclast precursors differentiation into osteoclast. Lysm‐Cre⁺ cells (GFP, green) from bone marrow were sorted out by flow cytometry after injection with MCC950 or vehicle. (A) Flow diagram of distinguishing Lysm‐Cre⁺ cells (GFP⁺). (B) The selected Lysm‐Cre⁺ cells were cultured with M‐CSF and RANKL and stained for TRAP activity. Same field of TRAP‐stained images (left panel), fluorescence images (right panel) and merged images of bright field and fluorescence images (middle panel) were shown. Yellow arrows show TRAP‐positive and Lysm‐Cre⁺ cells. (C) TRAP⁺ OC area and TRAP⁺ OC number were assessed. (D) The expression levels of OC‐related genes were determined by qPCR. All the results are representative of at least three independent experiments. Values are mean ± SD Two‐tailed unpaired Student's t‐test was performed. *P < .05 in the indicated groups