Figure EV2. Immunoblot validation of ALKAL2 induction of EGR1 and FOS at the protein level in IMR32 cells.

1. IMR32 cells were treated for 0, 1 and 6 h in the presence and absence of lorlatinib as indicated. Lysates were immunoblotted for EGR1 and FOS, as well as pERK as readout of ALKAL2‐induced ALK signalling.
2. IMR32 cells were treated for 0 or 1 h in the presence and absence of lorlatinib as indicated. Lysates were immunoblotted for pSRF and SRF, as well as pERK as readout of ALKAL2‐induced ALK signalling.