Figure EV4. Correlation between phosphoproteomic responses after ALKAL2 stimulation and ALK inhibition.

Differential phosphorylation was determined in NB1 cells in response to 1h ALKAL2 stimulation. Plots show correlations with log₂FC values determined after inhibiting CLB‐BAR or NB1 cell lines with the ALK TKI lorlatinib or crizotinib as indicated. The inset on top left of each plot shows the linear regression line in red with indication of Pearson correlation coefficient and P value. Dashed square shows the region that is shown by the main plot. Genes indicated in red show differential phosphorylation (log₂FC below 0.5) in all ALK TKI conditions. When phosphorylation was measured in multiple sites from the same protein, the site with the highest absolute response was used for the analysis. ALK TKI data were obtained from 2 independent studies as indicated (Emdal et al, 2018; Van den Eynden et al, 2018).