TABLE 4.
Transcriptional regulation of chemoreceptor and pathways genes in P. aeruginosa during biofilm formation and infection
| Gene cluster/ORF | Fold change(s)a |
|||||||
|---|---|---|---|---|---|---|---|---|
| Biofilm formation on abiotic surfaces (reference[s]) |
Virulence/in vivo infection (reference) |
|||||||
| Biofilm/planktonic (159, 189) | Planktonic/dispersed cells (159) | Anaerobic biofilm/planktonic (190) | Human sputum/in vitrob (191) | Human sputum/in vitrob (161) | Human burn wound infection/in vitro (191) | Mice lung infection/in vitro (192) | Human urine/in vitro (193) | |
| Gene cluster I (F6)c | –3.3/–3.7* | –3.0/–6.3* | –2.5/–4.1* | –2.2/–6.5* | ||||
| Gene cluster II (F7)c | –3.5/–12.5* | 4.3/13.1* | –3.9/–42.2* | –3.0/–25.0* | –4.6/–32.2* | –4.4/–21.4* | –5.7/–15.9* | –2.7/–4.5* |
| Gene cluster III (Wsp)c | 3.0/4.3* | –3.0/–5.4* | –2.3/–2.6* | |||||
| Gene cluster IV (Chp)c | –2.9/–3.0* | –2.8/–4.4* | –3.13/–12.5* | –2.5/–4.9* | ||||
| Gene cluster V (F6) | –2.8 | –5.4 | –3.65/–16.0* | –1.9 | ||||
| PA0176 (McpB) | –5.8 | 4.9/7.3† | –31.2 | –25.1 | –13.5 | –9.0 | –12.6 | –4.5 |
| PA0180 (McpA) | –3.9 | –5.1 | –4.6 | –4.3 | –5.7 | –2.7 | ||
| PA0411 (PilJ) | –3.2/–5.1† | –12.5 | 3.9 | |||||
| PA1251 | –5.7 | 8.9/9.3† | –2.9 | –2.6 | –20.8 | |||
| PA1423 (BdlA) | –3.6 | |||||||
| PA1561 (Aer) | –2.1/–7.9† | –1.5/–2.8† | –7.96 | |||||
| PA1608 | ||||||||
| PA1646 | 2.0 | 2.6 | ||||||
| PA1930 (McpS) | 20.8/31.2† | –9.3 | –33.2 | –7.9 | ||||
| PA2561 (CtpH) | 2.6 | 4.28 | 3.5 | |||||
| PA2573 | –3.1 | 12.7/22.1† | –28.8 | –6.4 | –12.2 | –2.8 | ||
| PA2652 (CtpM) | –2.5 | –4.7 | ||||||
| PA2654 (TlpQ) | –2.1 | |||||||
| PA2788 (McpN) | 3.0/5.6† | –7.4 | –8.2 | –12.4 | –4.1 | –10.9 | ||
| PA2867 | –2.1/–3.3† | –5.3 | –10.4 | –7.6 | –8.8 | |||
| PA2920 | –4.2 | 11.1/12.3† | –3.6 | –5.1 | –6.9 | –7.6 | ||
| PA3708 (WspA) | 4.3 | |||||||
| PA4290 | 4.6/13.4† | 4.2 | –2.0 | –31.8 | –21.8 | |||
| PA4307 (PctC) | –3.3/–7.4† | –3.2 | –2.9 | –2.0 | 9.61 | 2.2 | ||
| PA4309 (PctA) | –2.5 | –3.8 | –2.3 | –2.9 | –2.0 | |||
| PA4310 (PctB) | –4.3 | |||||||
| PA4520 | –3.1 | –2.6 | ||||||
| PA4633 | –2.6 | –2.9 | –2.8 | –3.6 | –2.8 | |||
| PA4844 (CtpL) | 3.7 | |||||||
| PA4915 | 3.6/7.9† | –24.1 | –3.8 | –5.7 | –2.2 | –6.0 | –2.8 | |
| PA5072 (McpK) | 3.0 | –2.5 | 5.2 | |||||
a
*, The lowest and highest fold change values among the differentially expressed genes per cluster are listed; †, values for two different studies are listed.
b
Human sputum refers to cystic fibrosis expectorated sputum.
c
At least two genes per gene cluster have to be altered for listing.