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. 2021 Feb 1;35(3-4):250–260. doi: 10.1101/gad.339903.120

Figure 5.

Figure 5.

Induction of MYC/MYCN by ROS. (A) Western blot analysis of MYC/MYCN in WT (20% and 1% O2), Hif1a KO (1% O2), and Nox1 KO (1% O2) GS cells (n = 3). (B) Proliferation of Hif1a KO GS cells (n = 3). Cell recovery was determined after culturing under hypoxia for 3 d. (C) Western blot analysis of MYC/MYCN in WT GS cells after exposure to H2O2, apocynin or LPA (n = 3). (D) Flow cytometric analysis of ROS levels by CellROX Deep Red after Mycn overexpression in WT GS cells 11 d after transfection (n = 3). (E) Flow cytometric analysis of ROS levels in Myc DKO GS cells by CellROX Deep Red. (F) Proliferation of Myc DKO GS cells (n = 3). Cells were recovered after 4 d. (G) Proliferation of Myc DKO GS cells transfected with Mycn (n = 3). Cells were recovered 8 d after transfection. Asterisk indicates statistical significance (P < 0.05).