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. 2021 Feb 1;35(3-4):218–233. doi: 10.1101/gad.344184.120

Figure 4.

Figure 4.

ATDC-mediated increase in NRF2 regulates growth and invasion in PDA cells in vitro. (A,E) Immunoblotting showing levels of ATDC, NRF2, KEAP1, and NRF2 target NQO1 in HPAC cells with ATDC knockdown with or without subsequent NRF2 overexpression (A) and S2-013 cells overexpressing ATDC with or without subsequent knockdown of NRF2 (E). (B–D) Cell proliferation (B), invasion (C), and ROS formation (D) in HPAC cells with or without sh ATDC and NRF2 rescue. (F–H) Cell proliferation (F), invasion (G), and ROS formation (H) in S2-013 cells with or without ATDC overexpression and sh NRF2 rescue. (I) Cell proliferation in ATDC overexpressing S2-013 or Capan2 or HPAC cells with ATDC knockdown treated with increasing concentrations of NAC (0–10 mM). (J) Western blot analysis of ATDC and NRF2 expression in S2-013 cells expressing full-length ATDC or the ATDCΔ220N deletion. β-actin was used as a loading control. (K–M) Quantification of cell proliferation (K), invasion (L), and ROS levels (M) in S2-013 cells expressing ATDC or ATDCΔ220N. All experiments were repeated three times. (*) P < 0.05, (**) P < 0.01, (***) P < 0.005. Mean ± SEM.