Figure 2.

Generation of conditional alleles for another four genes by the i-GONAD method. (A) Gene information: ¹coordinates as of mouse GRCm38/mm10 genome assembly; ²NCBI annotation; ³query of Tabula Muris single-cell RNA sequencing data. (N/A) Data not available. (B–E) Gene structures and relative positions of ssODNA, gRNA, and genotyping primer for Plagl1 (B), Ak040954 (C), Clcf1 (D), and Gm44386 (E) genes. For Plagl1, 5′- and 3′-loxP bands are 312 bp and 234 bp; for Ak040954, 5′- and 3′-loxP bands are 361 bp and 391 bp; for Clcf1, 5′- and 3′-loxP bands are 389 bp and 222 bp; for Gm44386, 5′- and 3′-loxP bands are 431 bp and 415 bp. Boxed panels showed sequencing verifications of loxP sites in F1 generations. (Het) Heterozygous.