FIG 7.

GLUT8 partly colocalizes with LAMP1 in lysosomes. (A) MB231 cells expressing C-terminal-FLAG-tagged GLUT8 (CF GLUT8) were stained with anti-GLUT8 and LAMP1; single stains are shown to the right, and colocalization is shown to the left; nuclei were stained with DAPI. (B) MB231 cells expressing untagged GLUT8, and cells without transgenic GLUT8 expression, are compared with the data shown in panel A, to assess the relative distribution of untagged GLUT8 protein, lysosome distribution, and endogenous GLUT8 localization. (C) For comparison, MB231 cells expressing GLUT1 were stained using the same protocol. Scale bars = 10 μm.