TABLE 2.
Antibodies used in this study
| Target | Sourcea | Working dilution (IHC)a |
|---|---|---|
| β-Actin | Mouse; Sigma no. A5441 | − |
| EEA1 | Rabbit; Thermo Fisher MA5-147494 | 1:1,000 |
| FLAG | Rabbit; Cell Signaling no. CS14793 | 1:800 |
| FLAG | Mouse; Sigma no. F3165 | 0.5 μg/ml |
| GLUT8 | Rabbit; Biobasic Inc.; BBA1 | 1:100 |
| GLUT1 | Rabbit; S. Anderson (UC Denver) | 0.2 ng/ml |
| LAMP1 | Mouse; DHSB no. H4A3 | 1:100 |
| PDI | Rabbit; Cell Signaling no. CD3501 | 1:1,000 |
| PMP70 | Rabbit; Invitrogen no. PA1-650 | 5 μg/ml |
| Vinculin | Mouse; Millipore no. 05-386 | − |
| Mouse | Goat; Alexa Fluor 546; Thermo Fisher no. A11030 | 1:500 |
| Rabbit | Goat; Alexa Fluor 488; Thermo Fisher no. A11034 | 1:500 |
| Mouse | Goat; Alexa Fluor 488; Thermo Fisher no. A11029 | 1:500 |
| Rabbit | Goat; HRP; Invitrogen, no. G-21234 | – |
| Mouse | Donkey; HRP; Jackson, no. 715-035-151 | – |
a
HRP, horseradish peroxidase; IHC, immunohistochemistry; –, not applicable.