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. 2021 Feb;31(2):171–185. doi: 10.1101/gr.263814.120

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© 2021 Collins et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Circadian regulation of metabolism and mitochondrial morphology leads to a peak in mitochondrial-linked oxygen consumption rate in the early phase. Seahorse-derived measurements at different time points for the rate of (A) basal respiration and (B) ATP-linked OCR, in picomoles/min for 50,000 cells/well and 30 total wells per time point. (****) P ≤ 0.0001. (C) The oscillation of MFF protein in the proteomics time course. Across circadian time in synchronized BMDMs, mitochondria were stained using MitoTracker Red CMXRos (D), and the amount of fragmented (E) and elongated (F) mitochondria were quantified by confocal microscopy by measuring the length of over 50 mitochondrial particles per cell for 25 cells per sample in triplicate independent time courses. Mitochondria with intermediate lengths were considered “tubular” and therefore neither elongated nor fragmented.