Table 1.

Sequences of primers and crRNAs for detection of SARS-CoV-2 based on different CRISPR-Cas12a assays.

No	Gene	Primer name	Sequence (5′-3′)	Reference
1	N1 gene	RT-AIOD-CRISPR F primer	AGGCAGCAGTAGGGGAACTTCTCCTGCTAGAAT	Ding et al. (2020)
		RT-AIOD-CRISPR R primer	TTGGCCTTTACCAGACATTTTGCTCTCAAGCTG
		crRNA1	UAAUUUCUACUAAGUGUAGAUCAUCACCGCCAUUGCCAGCC
		crRNA2	UAAUUUCUACUAAGUGUAGAUUUGCUGCUGCUUGACAGAUU
2	N2 gene	2019-nCoV_N2-F_RPA	ACAAGGAACTGATTACAAACATTGGCCGCAAA	Broughton et al. (2020)
		2019-nCoV_N2-R_RPA	TTCCATGCCAATGCGCGACATTCCGAAGAA
		N-gene gRNA	UAAUUUCUACUAAGUGUAGAUCCCCCAGCGCUUCAGCGUUC
3	E gene	E-Sarbeco_F1_RPA	GAAGAGACAGGTACGTTAATAGTTAATAGCGT	Broughton et al. (2020)
		E-Sarbeco-R2_RPA	ACGTTAACAATATTGCAGCAGTACGCACACA
		E-gene gRNA	UAAUUUCUACUAAGUGUAGAUGUGGUAUUCUUGCUAGUUAC
4	S gene	SARS2_spike1-F	CCACTGAGAAGTCTAACATAATAAGAGGCTG	Mayuramart et al. (2020)
		SARS2_spike1-R	AATAAACTCTGAACTCACTTTCCATCCAACT
		SARS2_spike2-F	AATCTATCAGGCCGGTAGCACACCTTGTAAT
		SARS2_spike2-F	TCCACAAACAGTTGCTGGTGCATGTAGAAGTT
		SARS2-S1	UAAUUUCUACUAAGUGUAGAUGAUUCGAAGACCCAGUCCCU
		SARS2-S2	UAAUUUCUACUAAGUGUAGAUCAAUCAUAUGGUUUCCAACC

Note: Underline sequence represents spacer sequence.