Figure 1.

Tissue factor production after polarization of macrophages. (A) Tissue factor (TF) protein was determined on extracellular vesicles from supernatant (n=6) and (B) from lysed cells (n=7) using a specific enzyme-linked immunosorbent assay as indicated in the Methods section. (C) TF activity on extracellular vesicles from polarized macrophages was evaluated using an activity assay as indicated in the Methods section (n=13). Values are given in pg/mL and represent mean values ± standard deviation. (D) Capability of human polarized macrophages to form filopodia when migrating onto laminin-coated areas was evaluated by cytoskeletal staining (n=3). M0: unpolarized macrophages; M(LPS+IFN): classically activated polarized macrophages; M(IL-4+IL13): alternatively activated polarized macrophages; ns: not significant.