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. 2020 Jan 23;106(2):454–463. doi: 10.3324/haematol.2019.220210

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Copyright© 2021 Ferrata Storti Foundation

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Figure 6. — Staining of tissue factor-positive macrophages in sections of colon carcinoma. (A) Tissue factor (TF) (red) and CD206 (green) were stained in colon cancer tissue using specific antibodies as described in the Methods. CD206⁺ macrophages positive for TF are indicated with white arrows. The boxed area is shown in more detail. (B) White arrows indicate acellular regions that showed double staining for CD206 and TF (orange), which could represent extracellular vesicles derived from macrophages positive for CD206 and TF. (C) Distribution of CD206⁺ macrophages was evaluated and scored in areas with low, medium, and high TF density. (D) Human atherosclerotic plaque tissue was stained for TF (green) and either CD206 (red) alternatively activated macrophages or CD80 (red) for proinflammatory macrophages. Adjusted TF intensity to macrophage intensity demonstrated an increase in TF in CD206⁺ regions. Values are given as adjusted tissue factor intensity (arbitrary units) mean values ± standard deviation (SD) (n=16 patients). (E, F) Mouse macrophages from atherosclerotic plaques were isolated as indicated in the Online Supplement. Proinflammatory macrophages were less positive for TF and showed reduced mean fluorescence intensity compared to alternatively activated CD206 macrophages (n=11). Values represent mean values ± SD. DAPI: 4',6-diamidino-2-phenylindole; a.u.: arbitrary units.