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. 2021 Jan 18;11:623190. doi: 10.3389/fphys.2020.623190

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Copyright © 2021 Pérez-Hernández, Marrón-Liñares, Schlamp, Heguy, van Opbergen, Mezzano, Zhang, Liang, Cerrone and Delmar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation of Ribo^flox/flox/PKP2^{flox/flox/Cre+} mice. (A) RiboTag^flox/flox/-PKP2^flox/flox mice were obtained crossing RiboTag mice (JAX B6N.129-Rpl22/J 011029) with PKP2cKO/αMyHC-Cre-ER(T2). Cre + and Cre- mice were tamoxifen-injected and used 21 days post injection (dpi). (B) Mice hearts were analyzed by western blot. The 23-kDa 3HA epitope-tagged ribosomal protein RPL22 was detected by an HA antibody. A heterozygous RiboTag^flox/wt was used. For experiments, we used homozygous RiboTag^flox/flox to increase 3HA-RPL22 expression and facilitate breeding scheme.