(A) Representative examples of TM cells immunolabeled for vinculin. Scale bar is 50 μm. (B) Bar graphs summarizing effects of shear stress and GsMTx4 on a number of vocal adhesions. ###p < 0.00001; n = 112, 120, 120 and 108 cells for control, shear, GsMTx4, and shear+GsMTx4 groups, respectively. ANOVA multiple comparisons test, Tukey’s test was applied for means comparison, R-Square = 45 %. Shown are the mean ± SD. GsMTx4 (5 μM) was added to the perfusing solution 5 min prior to shear stress.