Fig. 2.

Expression of Mbd2, Mbd3, and Mecp2 in SHS exposed mice versus control. The transcription level of genes encoding major MBDs was examined in T⁴and T⁴⁺⁷ mice by qRT-PCR using the 2^−ΔΔCt method [41]. Bars represent the mean normalized expression (±SD) of triplicate samples in SHS-exposed mice and age-matched controls. Data were normalized using the endogenous housekeeping gene, Gapdh, as reference and controls as calibrators. Marginal differences in transcription levels between SHS-exposed and control mice were not statistically significant (Fisher’s exact test).