Figure 1.

PRR9, 7 in roots inhibit EC complex gene expression under warm temperature. Transcript accumulation of ELF3 (a), ELF4 (b), and LUX (c) were examined in prr7 prr9 mutant and wild-type plants. The seedlings were grown under 12:12 LD cycles at 22°C for 14 days. After the shoots and roots were separated, they were transferred to 22°C or 28°C, respectively, under constant light conditions. Samples were harvested every 3 h over a 24-h cycle for RNA extraction and qRT-PCR analysis. The reference gene IPP2 was used in the quantitative expression analysis. The data are presented as the mean ± SEM of three technical replicates from one of three independent biological experiments; all experiments yielded congruent results. White or gray bars represent subjective day or subjective night, respectively