Figure 2.

The negative regulation of PRR9, 7 in roots on GI is weakened at warm temperature. Transcript levels of GI were analyzed at 22°C and 28°C, respectively. Arabidopsis seedlings of the prr7 prr9 mutant and wild-type plants were grown under 12:12 LD cycles at 22°C for 14 days. After the shoots and roots were separated, they were transferred to 22°C or 28°C, respectively, under constant light conditions. Samples were harvested every 3 h over a 24-h cycle for RNA extraction and qRT-PCR analysis. The reference gene IPP2 was used in the quantitative expression analysis. The data are presented as the mean ± SEM of three technical replicates from one of three independent biological experiments; all experiments yielded congruent results. White or gray bars represent subjective day or subjective night, respectively