Figure 1.

miR-20a is overexpressed while CDH1 is suppressed in brain tissues of rats with cerebral I/R injury. (a). Changes in miR-20a and CDH1 mRNA expression in rat brain tissues were determined by RT-qPCR (n = 6); (b). Changes in CDH1 protein content in rat brain tissues were determined by western blot analysis (n = 6); (c). miR-20a and CDH1 binding site predicted by online website; (d). Targeting relationship between miR-20a and CDH1 was verified by luciferase activity assay; a P < 0.05 compared with the sham group; b P < 0.05 compared with the inhibitor NC group; c P < 0.05 compared with the OE-NC group; d P < 0.05 compared with the miR-20a inhibitor + si-NC group; in Figure (d), data were compared by t-test; data were analyzed by one-way ANOVA while pairwise comparison after one-way ANOVA by Tukey’s multiple comparisons test