Table 1.
Gene | Primer sequence |
---|---|
miR-20a | Forward: 5’-GCTGCCGTAAAGTGCTTATAGTG-3’ |
Reverse: 5’-AGAGCAGGGTCCGAGGTA-3’ | |
U6 | Forward: 5’-CGCTTCGGCAGCACATATAC-3’ |
Reverse: 5’-AAATATGGAACGCT-TCACGA-3’ | |
CDH1 | Forward: 5’-TGAGGTCGGTGCCCGTATTG-3’ |
Reverse: 5’-TCGTTGGTCTTGGGGTCTGT-3’ | |
GAPDH | Forward: 5’-ACGGCAAGTTCAACGGCACAG-3’ |
Reverse: 5’-GACGCCAGTAGACTCCACGACA-3’ |
Note: miR-20a; microRNA-20a; CDH1, Cadherin 1; GAPDH; glyceraldehyde-3-phosphate dehydrogenase.