TABLE 1.
Primers used in this study
| PCRs flanking nonoverlapping contigs | Primer namea | Sequence (5′–3′) | PCR Tm (°C) | PCR product length (bp) | PCR extension time (min:s) |
|---|---|---|---|---|---|
| PCR 1 | 5121 | TATCAATAACGATAGTATGCCCAGCG | 65 | 4,712 | 2:21 |
| 3121 | TTCCAATAGGTCTTGATAGTGAGGTG | ||||
| PCR 2 | 5122 | GTTTGTTGCAGTCGTCGTTATCTC | 63 | 3,392 | 1:42 |
| 3122 | CGTCGGAATTAAATCTTGGAATACC | ||||
| PCR 3 | 5123 | GGCATCAGGAATGATCTGATTTACAC | 65 | 2,064 | 1:02 |
| 3123 | TGCCTCCCATTGGATTACTGTATAC | ||||
| PCR 4 | 5124 | GACTCGATAGGGTATATGGTGCC | 65 | 5,126 | 2:34 |
| 3124 | GGTTCGATTGCGTTACTGCG | ||||
| Sequencing primers | |||||
| PCR 1 | 5131 | GTGACATAGATTGGAATAGGGTTAGC | |||
| 5132 | TATTCTCAGTGTCTGTGTACTACTGC | ||||
| 5133 | AAATCTTGGCAGACAGTGGTTATC | ||||
| 5134 | CAACAGGAGGAACCTGTAGAAGTTC | ||||
| 5135 | TACAATCCATCTCTGGAATTCAC | ||||
| 3131 | GTGACATAGATTGGAATAGGGTTAGC | ||||
| 3132 | ATAATAAGGTGTCAGACAAACTCGC | ||||
| 3133 | GGTTCGTCATTTATGAATGGTGTAC | ||||
| 3134 | TTGACTATGGTTATGCTTTCAGG | ||||
| 3135 | TTCTCAACCTTGATTCTCTCTTTGG | ||||
| 3136 | GTGCCGTTTCAAAGGTCGCT | ||||
| 3137 | CCGGGCTCGCTCCATATAGATAAG | ||||
| PCR 2 | 5136 | TATGCTCTCATAGGTAACACCACC | |||
| 5137 | AACGATCACCTAAATTAGTACCTGC | ||||
| 5138 | TCTATCTTGTTCCTGTTTCCTTG | ||||
| 5139 | TTTAGGTTAGAAAGGAGATACTGCC | ||||
| 5140 | TACTTCAAATGGTATGCAAGCTATGG | ||||
| 3138 | GCTGAACAAGCTGCTGTTATTGC | ||||
| 3139 | TTTAGTTGAGGATGCTTATCGAG | ||||
| 3140 | AGTTATCTGTCTATAAGGAATGTCG | ||||
| 3141 | AAGCTATGGTTGAAGCTGTTG | ||||
| PCR 3 | 5141 | TTAAATTAACTCCTGAAGTACTCCG | |||
| 3142 | AGGTAATTTCCATTTCTCACCTGAAG | ||||
| 3143 | TTTCGGCGACAATTCATTGAACTGAG | ||||
| PCR 4 | 5125 | GTAACTAGTTATCTCTAGCCTGTAGC | |||
| 5126 | CACGAAAGCAACTTAATCCGTCG | ||||
| 5127 | CCCTTGACTACATAAGTACTAACCC | ||||
| 5128 | CTGTTAATAAATCAGCTCCATGAGC | ||||
| 5129 | TTTCCCTTGCATTTCCCATAGACC | ||||
| 5130 | GCCTATCCAATTATTCGTTTGGAG | ||||
| 3125 | TTTACCTCTGTTGCATCCACAATC | ||||
| 3126 | GCAAAGCAATTGTATTCCGTCTT | ||||
| 3127 | AAAGTGTCGTTACCAACTCTGAAG | ||||
| 3128 | AAATTATGAATCAGGCATGCTCCTGG | ||||
| 3129 | AATAAAGCCTGAAACCAGTTCAGAG | ||||
| 3130 | CATCACTCTGGCCTCTATTATTT |
a
Primer names beginning with 5 indicate forward primers. Primer names beginning with 3 indicate reverse primers.