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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: J Am Chem Soc. 2016 Apr 19;138(16):5392–5402. doi: 10.1021/jacs.6b01989

Figure 2.

Figure 2.

A. CWEPR spectra of γS-WT (green) and γS-G18V (blue) at low concentration (~10mg/ml). B. Representative CWEPR spectra of γS-G18V in the monomeric state (I.), with onset of aggregation (II.), and upon aggregation (III.). C Representative CWEPR spectra of γS-WT stored below ~100 mg/ml prior to spin labeling show mobile spin labels at 5 and 270 mg/ml, respectively (IV,V). A broader line (VI.) is observed when a threshold concentration (>100 mg/ml) for γS-WT is reached prior to spin labeling, as observed for protein concentrations between 5–550 mg/ml (see Section 2 in SI).