Figure 2.
Elevation of H19, HMGB1 and reduction of miR-129 exhibit in PC12 cells after induction of Aβ25-35 A. H19, miR-129, HMGB1 expression in PC12 cells treated with different concentrations of Aβ25-35 detected via RT-qPCR; B. HMGB1 protein bands in PC12 cells treated with different concentrations of Aβ25-35; C. HMGB1 protein expression in PC12 cells treated with different concentrations of Aβ25-35 detected via western blot analysis; D. H19, miR-129, HMGB1 expression in Aβ25-35-treated PC12 cells after transfection detected via RT-qPCR; E. HMGB1 protein bands in Aβ25-35-treated PC12 cells after transfection detected via western blot analysis; F. HMGB1 protein expression in Aβ25-35-treated PC12 cells after transfection detected via western blot analysis. The data in the figure were all measurement data, in the form of mean ± standard deviation. Student’s t test was used to evaluate significant differences between two groups of data. One-way ANOVA was functioned for comparison among multiple groups. The pairwise comparison after ANOVA analysis used Tukey’s post hoc test, N = 3, In the A and C, + vs 0 μmol/L, P < 0.05; In the D and F, * vs the si-NC group, P < 0.05; # vs the mimic NC group; & vs the OE-H19 + mimic NC group, P < 0.05