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Palbociclib treatment induces senescence in TSC1 –/– MEFs. TSC1+/+ and TSC1 –/– MEFs were treated with vehicle only (-) or indicated palbociclib concentration for 7 days and subjected to western blotting analyses of the indicated proteins (A) or SA-β-Gal activity (B). Representative images of SA-β-Gal stained samples captured with 10× objective are shown. Scale bars, 100 µm
