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. 2019 Jul 3;12(2):114–121. doi: 10.1080/21541248.2019.1635403

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This work was authored as part of the Contributor’s official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.

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Figure 2. — Glucose promotes translocation and membrane association of Rac1, but not RhoGDIβ, in INS-1 832/13 cells. INS-1 832/13 cells were exposed to low glucose (LG, 2.5 mM) or stimulatory glucose (HG, 20 mM) for 15 min. Cell lysates were subjected to subcellular fractionation and analyzed for RhoGDIβ and Rac1 protein expression levels in cytosolic and membrane fractions. GAPDH and E-Cadherin were used as loading controls for cytosol and membrane fractions, respectively. Representative blots from three independent studies are provided. (b) Densitometric quantitation of relative abundance of Rac1 and RhoGDIβ in the membrane fractions isolated from low and stimulatory glucose-treated INS-1 832/13 cells (a) is shown here. The data are expressed as fold change relative to LG-treated. (mean ± SEM; n = 3; * p < 0.05)