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. 2019 Jul 3;12(2):114–121. doi: 10.1080/21541248.2019.1635403

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This work was authored as part of the Contributor’s official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.

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Figure 3. — Glucose-induced translocation and membrane association of Rac1 is not altered in RhoGDIβ -depleted (RhoGDIβ siRNA) INS-1 832/13 cells. INS-1 832/13 cells were transfected with scrambled siRNA or siRNA targeted to RhoGDIβ. Cell lysates prepared after 48 h were analyzed by Western blotting for the expression of RhoGDIβ (a) or RhoGDIα (b; as a negative control to assess off-target effects). Actin was used as loading control. A representative blot from n = 3 independent studies is shown here. (c) Western blot data indicating that glucose-induced membrane association of Rac1 was not affected following RhoGDIβ depletion in INS-1 832/13 cells. (d) Densitometric quantitation of Rac1 expression in the membrane fraction depicted in Panel C is shown here. The results are presented as means ± SEM. The data are expressed as fold change relative to LG-treated Scr-si. (n = 4; * p < 0.05). Comparisons shown: a: significant compared with LG-treated Scr-si; b: significant compared with LG-treated RhoGDIβ-si