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. 2019 Jul 3;12(2):114–121. doi: 10.1080/21541248.2019.1635403

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This work was authored as part of the Contributor’s official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.

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Figure 4. — Glucose-induced Rac1 activation is attenuated in INS-1 832/13 cells following suppression of RhoGDIβ expression. a. INS-1 832/13 cells were transfected with scrambled siRNA or siRNA targeted to RhoGDIβ protein. After 48 h of transfection, cells were subjected to overnight starvation and then were treated with LG (2.5 mM) or HG (20 mM) for 15 min. Rac1 activation was quantified by Rac1 pull-down assay. Representative blots from four independent studies are provided. (b) Densitometric quantitation of activated Rac1 in Panel A is shown here. The results from four independent experiments are presented as means ± SEM. The data are expressed as fold change relative to LG-mock (n = 4; * p < 0.05). Comparisons shown: a: significant compared with LG-treated mock; b: significant compared with HG-treated mock; c: significant compared with LG-treated Scr-si. d: significant compared with HG-treated Scr-si; NS: Not significant