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. 2019 Jul 3;12(2):114–121. doi: 10.1080/21541248.2019.1635403

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This work was authored as part of the Contributor’s official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.

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Figure 5. — siRNA-mediated knockdown of RhoGDIβ modestly, but significantly, potentiates GSIS in INS-1 832/13 cells. A. INS-1 832/13 cells were transfected with scrambled siRNA or siRNA targeted to RhoGDIβ protein. After 48 h of transfection, cells were subjected to overnight starvation and then GSIS was quantified by ELISA as described under Methods. Data are mean ± SEM from four experiments. The data are expressed as fold change relative to LG-mock. (n = 4; * p < 0.05). Comparisons shown: a: significant compared with LG-treated mock; b:significant compared with LG-treated Scr-si; c: significant compared with LG-treated RhoGDIβ-si; d: significant compared with HG-treated Scr-si. NS: not significant. A representative blot demonstrating the efficiency of RhoGDIβ knockdown is shown in Panel B.