Figure 4.

Compound screening for small molecule ligands of Fgd5. The Fgd5 triple domain was crosslinked to a CM5 Biacore chip and compounds from the LOPAC¹²⁸⁰ library were screened for binding by surface plasmon resonance (SPR). (a) Representative SPR curves for compounds that showed no response (N-acetyl-L-cysteine) and high response (mitoxantrone, dequalinium chloride and aurintricarboxylic acid). Response calculations from compounds showing greater than 3-fold response over the average response calculated for each plate are provided in Table S1 (supplemental data). (b) Structures of the top 11 compounds selected for further analysis