Table 6.
Whey permeate1, % | P-value | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Item | 0 | 3.75 | 7.50 | 11.25 | 15.00 | 18.75 | SEM | Linear | Quad. | 0% vs. others |
Diversity | ||||||||||
Chao1 | 97.4 | 132.2 | 104.2 | 103.2 | 107.0 | 92.1 | 21.4 | 0.569 | 0.543 | 0.663 |
Shannon | 3.14 | 3.28 | 3.47 | 2.54 | 3.36 | 3.00 | 0.60 | 0.784 | 0.993 | 0.984 |
Simpson | 0.69 | 0.69 | 0.76 | 0.63 | 0.76 | 0.73 | 0.08 | 0.678 | 0.920 | 0.774 |
Phylum level | ||||||||||
Actinobacteria | 5.65 | 4.21 | 5.97 | 5.23 | 6.24 | 5.28 | 0.91 | 0.595 | 0.934 | 0.753 |
Bacteroidetes | 21.92 | 21.93 | 19.59 | 23.07 | 21.69 | 22.55 | 2.19 | 0.779 | 0.729 | 0.954 |
Firmicutes | 31.39 | 25.64 | 33.15 | 26.65 | 28.13 | 30.04 | 5.05 | 0.498 | 0.537 | 0.598 |
Proteobacteria | 17.11 | 17.75 | 17.71 | 15.63 | 18.3 | 21.06 | 2.49 | 0.363 | 0.366 | 0.722 |
F:B ratio | 1.59 | 1.19 | 1.35 | 1.25 | 1.22 | 1.13 | 0.17 | 0.144 | 0.650 | 0.073 |
Family level | ||||||||||
Acetobacteraceae | 4.16 | 3.34 | 3.31 | 4.33 | 4.19 | 5.91 | 1.06 | 0.199 | 0.270 | 0.965 |
Bacillaceae | 0.92 | 0.95 | 0.84 | 0.97 | 1.89 | 0.67 | 0.27 | 0.475 | 0.417 | 0.638 |
Bifidobacteriaceae | 0.73 | 0.55 | 0.83 | 0.81 | 0.97 | 1.11 | 0.22 | 0.089 | 0.599 | 0.607 |
Clostridiaceae | 2.20 | 1.31 | 1.66 | 2.11 | 2.58 | 1.27 | 0.47 | 0.922 | 0.695 | 0.375 |
Corynebacteriaceae | 0.40 | 0.96 | 0.71 | 0.69 | 1.11 | 0.43 | 0.28 | 0.742 | 0.090 | 0.131 |
Enterobacteriaceae | 1.04 | 0.66 | 0.62 | 0.49 | 0.51 | 0.52 | 0.20 | 0.091 | 0.266 | 0.036 |
Helicobacteraceae | 8.37 | 9.11 | 6.47 | 9.02 | 7.62 | 7.31 | 2.63 | 0.723 | 0.990 | 0.862 |
Lachnospiraceae | 4.85 | 4.09 | 4.27 | 4.79 | 4.79 | 2.69 | 1.09 | 0.341 | 0.660 | 0.480 |
Lactobacillaceae | 6.94 | 7.03 | 12.66 | 12.81 | 9.19 | 8.82 | 1.48 | 0.182 | 0.010 | 0.052 |
Methylobacteriaceae | 1.10 | 2.15 | 2.23 | 1.94 | 1.82 | 1.55 | 0.46 | 0.810 | 0.114 | 0.139 |
Prevotellaceae | 7.25 | 7.99 | 7.08 | 7.08 | 6.68 | 6.73 | 4.75 | 0.298 | 0.586 | 0.772 |
Propionibacteriaceae | 1.77 | 1.39 | 2.27 | 1.85 | 2.41 | 1.74 | 0.71 | 0.687 | 0.685 | 0.840 |
Rhodobacteraceae | 5.92 | 2.61 | 2.64 | 3.08 | 4.35 | 5.83 | 1.19 | 0.609 | 0.015 | 0.101 |
Ruminococcaceae | 2.44 | 2.20 | 1.75 | 2.43 | 2.42 | 0.98 | 0.61 | 0.271 | 0.488 | 0.476 |
Streptococcaceae | 1.50 | 1.87 | 0.82 | 0.92 | 0.67 | 0.71 | 0.51 | 0.094 | 0.743 | 0.380 |
1Treatments were the inclusion levels of whey permeate in the diets.