Skip to main content
. 2020 May 6;143(6):1697–1713. doi: 10.1093/brain/awaa116

Figure 2.

Figure 2

Behavioural recovery of spinal cord-injured monkeys increase by NgR-Fc treatment. (A–C) Quantifications of open field voluntary feeding behaviour before and 6 months after SCI. (A) Hand usage preference 1 week before SCI. In a 10-min video analysis, the number of times that the animal uses its left hand, right hand (lesion side), or both hands to bring food to its mouth was counted. The hand usage preference was normalized to the control side (left hand, serving as internal control). (B) Hand usage preference 6 months after SCI. The attempts to use the right hand or both hands increased significantly for animals in the NgR-Fc-treated group (P = 0.047 by unpaired two-tailed t-test with Welch’s correction). Mean ± SEM with individual animals indicated. (C) Time course of hand usage preference as a function of time relative to SCI. The pre-injury and 6-month data from A and B are included in this replot. Mean ± SEM, n = 6 for control and n = 7 for NgR-Fc. By mixed-effects model, there was a significant interaction of time and treatment, P = 0.034, and by unpaired two-tailed t-test with Welch’s correction at 6 months, P = 0.047 for treatment as in B. (D–I) Hindlimb functional recovery after NgR-Fc treatment. (D) In the open field video analysis of movement assay, hip, knee and ankle movements were scored 6 months after SCI. The NgR-Fc treatment group shows significant recovery of their joint’s movements in the hindlimb walking assessment (P = 0.023 by unpaired two-tailed t-test with Welch’s correction). (E and F) Comparison of hindlimb walking scores at 1 week pre-injury and 6 months post-injury for each individual monkey. There was a statistically significant difference for animals from the NgR-Fc treatment group (P = 0.0017 by unpaired two-tailed t-test with Bonferroni-Dunn correction for multiple tests) and no significant difference for animals from the vehicle treatment group (P = 0.07). Mean ± SEM with individual animals indicated. (G) Quantification of the hindlimb digital function. There was a trend of increasing hindlimb digital scores in the NgR-Fc-treated group compared to the vehicle-treated group, but no significant statistical difference. (H and I) Comparison of hindlimb digital function scores at 1 week pre-injury and 6 months post-injury for each individual monkey. There was a statistically significant difference for animals from the NgR-Fc treatment group (P = 0.0065 by unpaired two-tailed t-test with Bonferroni-Dunn correction for multiple tests) and no significant difference for animals from the vehicle treatment group (P = 0.07). Mean ± SEM with individual animals indicated.