Figure 6.

Expression of hepatic di-and triglyceride synthesis genes are increased in Mrp4 KO mice. A, mRNA expression of genes involved in hepatic di-and triglycerides synthesis such as lipin-1, Dgat1 and 2 were analyzed using RT-qPCR. Hepatic lipin-1 protein expression in cytosolic (B) and nuclear fractions (C) was analyzed using Western blot analysis. A total cell protein lysate of HEK-293 cells overexpressing HA-tagged lipin-1 was used as positive control. Data are presented as mean ± SEM (p ≤ .05). An asterisk “*” denotes significant difference between WT and Mrp4 KO mice.