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. 2021 Jan 27;13:805–814. doi: 10.2147/CMAR.S285909

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© 2021 Jin et al.

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MiR-183-5p overexpression promotes the proliferation, migration, and invasion capacity of osteosarcoma cells. (A) Expression of miR-183-5p detected in HOS and U2OS cells after transfection with miR-183-5p mimics and negative controls. (B) Effect of miR-183-5p overexpression on the proliferation of HOS and U2OS cells assessed by CCK-8 assays. (C) Effect of miR-183-5p overexpression on the proliferation of HOS and U2OS cells assessed by crystal violet assays; the OD values of crystal violet assays was shown in right panel. (D) Effects of miR-183-5p overexpression on the migration of HOS and U2OS cells assessed by transwell assays (magnification: 400×); the calculation of cells that migrated through the filter was shown in right panel. (E) Effects of miR-183-5p overexpression on the invasion of HOS and U2OS cells assessed by transwell assays (magnification: 400×); the calculation of cells that invaded through the filter was shown in right panel. **P< 0.01; ***P< 0.001. Data are presented as mean ± SD of three independent experiments.