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. 2021 Jan 25;43(1):291–301. doi: 10.1080/0886022X.2021.1871922

Figure 2.

Figure 2.

miR-34b-5p promotes LPS-induced apoptosis and inflammatory response in HK-2 cells. Notes: (A) qRT-PCR detected the expression of miR-34b-5p in LPS-induced HK-2 cells; **P < 0.01, compared to the control group. After transfection of miR-34b-5p inhibitor or miR-34b-5p mimic in LPS-induced HK-2 cells, (B) qRT-PCR detected the expression of miR-34b-5p in HK-2 cells; (C) TUNEL staining measured the apoptosis rate of HK-2 cells; (D) Western blotting detected the expressions of Bax, Bcl-2 and cleaved caspase-3 in HK-2 cells; ELISA measured the levels of TNF-α (E), IL-1β (F), and IL-6 (G) in the supernatant of HK-2 cells; scale bar = 50 μm; *P < 0.05, **P < 0.01, ***P < 0.001, compared to the control group, LPS + inhibitor NC group or LPS + mimic NC group. LPS: lipopolysaccharide.