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. 2021 Jan 25;43(1):291–301. doi: 10.1080/0886022X.2021.1871922

Figure 3.

Figure 3.

Overexpressing AQP2 suppresses LPS-induced apoptosis and inflammatory response in HK-2 cells. Notes: qRT-PCR (A) and Western blotting (B) detected the expression of AQP2 in LPS-induced HK-2 cells; **P < 0.01, compared to the control group. After transfection of pCDNA3.1-AQP2 in LPS-induced HK-2 cells, qRT-PCR (C) and Western blotting (D) detected the expression of AQP2 in the cells; (E) TUNEL staining measured the apoptosis rate of HK-2 cells; (F) Western blotting detected the expressions of Bax, Bcl-2 and cleaved caspase-3 in HK-2 cells; ELISA measured the levels of TNF-α (G), IL-1β (H). and IL-6 (I) in the cell supernatant; scale bar = 50 μm; *P < 0.05, **P < 0.01, ***P < 0.001, compared to the LPS + pcDNA3.1 group. LPS: lipopolysaccharide.