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. 2021 Jan 25;43(1):291–301. doi: 10.1080/0886022X.2021.1871922

Figure 5.

Figure 5.

miR-34b-5p mediates LPS-induced apoptosis and inflammatory response in HK-2 cells by targeting AQP2. Notes: qRT-PCR (A) and Western blotting (B) detected the expression of AQP2 in LPS-induced HK-2 cells transfected with si-AQP2. After transfection of miR-34b-5p inhibitor + si-NC or miR-34b-5p inhibitor + si-AQP2 in LPS-induced HK-2 cells, (C) TUNEL staining assessed the cell apoptosis; (D) Western blotting measured the expressions of Bax, Bcl-2, and cleaved caspase-3 in HK-2 cells; ELISA measured the levels of TNF-α (E), IL-1β (F), and IL-6 (G) in the cell supernatant; scale bar = 50 μm; *p < 0.05, **p < 0.01, compared to LPS + inhibitor NC + si-NC group or LPS + inhibitor + si-NC group. LPS: lipopolysaccharide.